European Network on NMR Relaxometry

My previous researches were focused on examining signaling pathways involved in neurodegeneration neurons and exploring a role of isoforms PLA2 (cytosolic, inducible and secretary PLA2) in Alzheimer and Parkinson Disease pathology. These studies included amyloid beta, nitric oxide synthase, guanylyl cyclase, protein kinases, second messenger’s (NO, cGMP) and free radicals. My current researches are focused on: -Investigation effects of active pharmaceutical ingredients on the muscles parameters during and after activity, -Relations between biochemical markers, oxidative status, pain threshold and physical exercises, -Supplementation of polly unsaturatef fatty acids (PUFA) and their influence on cardiovascular system and muscles regeneration after exercise, -Physically active people nutrition and supplementation, -Skin parameters analysis and their dependences to physical exercises, -Analysis of electro-stimulation effect of smooth muscles with Body Flow therapy on some biochemical markers, oxidative status and pain threshold in relations with exercises. In my studies, I am using a wide range of laboratory techniques as: Western Blotting, ELISA test, DNA and RNA isolation, Real Time – PCR, electrophoresis and culture cell lines. I am also working in physical activity laboratory and do researches with trained people and measure some pulmonary and blood parameters and I estimate the level of aerobic and anaerobic efficiency. I cooperate with Medical University of Silesia and perform some researches and analysis: In my current studies the acquisition of image data has been supported by image analysis and processing methods, which allowed for their quantitative analysis. Thus, the results of these studies can be used for quantitative analysis of the effect of exercise on the tissue. The use of imaging methods also allows for the quantitative study of dosage forms (e.g PUFA supplementation), including the analysis of the distribution of the active pharmaceutical ingredient (API) and excipients with respect to the surface and / or volume of a dosage form. Distribution of the API and excipients in a dosage form is a critical parameter determining its efficacy and safety. Especially in this field - the impact of the excipients on API, I see a large possibility of application NMR relaxometry.